Basic Methodologies

  • Human embryo thawing: preblastocysts and blastocysts
  • Embryo culture up to blastocyst stage 
  • Inner cell mass isolation from blastocysts 
  • Feeder layer preparation for stem cell culture 
  • Human embryonic stem cell culture 
  • Detection of pluripotentiality markers by immunofluorescence 
  • G-banding 
  • DNA profile
  • Three germ lines differentiation capacity (in vivo and in vitro)
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