Human embryonic stem cell development in xeno-free conditions and in vivo characterization of their pluripotentiality.
This project addresses stem cell derivation methodology in order to adapt it to a xenobiotic-free system, so that derived stem cells may be useful for clinical applications. In that respect, the in vivo characterization of their differentiation potential is important to assure the biological properties of the in vitro cultures.
Human embryonic stem cell development from abnormal embryos and in vivo characterization of their pluripotentiality
This project proposes the use of embryos discarded for reproductive purposes after preimplantation genetic diagnosis due to their genetic or chromosomal abnormalities. In most cases these embryos will be carriers of monogenic disorders, so the aim of the project is to derive stem cell lines which will be used as models for diseases such as cystic fibrosis or haemophilia. Their banking at the BLCB and their availability for researchers will greatly help the advancement in the study of those diseases.