Zebrafish (Danio rerio) are small tropical fresh water fish native to the southeastern Himalayan region. Its high genetic homology to humans, the ease to genetically manipulate its transparent embryos and its regenerative capacity, are among the unique characteristics that make the zebrafish a valuable vertebrate model for studying human development, genetics and disease.
The Aquatic Animals Unit allows researchers to take advantage of these unique features offering zebrafish-based services and providing researchers with scientific and technical support to optimize their research projects.
Our services include:
•Morpholino, RNA o DNA microinjection
•In situ Hybridization
•RNA/ DNA extraction from embryos
•Organ/ tissue dissection from adult zebrafish
•Cardiac and fin surgery for regeneration studies
•Wild type, mutant and transgenic fish lines are available to provide either live or fixed embryos and larvae for in situ hybridization and immunohistochemical analyses
Our laboratory is fully equipped for standard fish work and has a microinjection station with:
•Sutter P-97 Needle Puller
•Eppendorf FemtoJet microinjector
•Olympus SZX-FO stereomicroscope
•Leica MZ16F Fluorescence Stereomicroscope with color digital camera and computer
•Incubator for embyo rearing