Personnel

Flow Cytometry

Our Flow Cytometry facility provides CMR[B] researchers with state-of-the-art equipment for flow cytometry and high-speed cell sorting. This unit works in a tight collaboration with the Cell Culture facility and the Stem Cell Bank to adapt new protocols of cell purification and characterization for stem cell research. The core equipment of this facility is a MoFlo cell sorter. This modular flow cytometer from Dako is equipped with three lasers (blue 488nm solid state laser, 635nm red diode and an Argon 351nm UV laser), 9 fluorescence detectors and 4-way sorting option.

Our MoFlo configuration provides the sorting accuracy needed to isolate different cell populations from a heterogeneous culture, tissue or organ. This cell sorter stands out for being a modular, flexible and versatile system providing researchers the possibility of setting the best instrument configuration that best fit every specific experiment or application. In addition of giving the user a high-speed cell sorting capacity it provides purities higher than 98% coupled with an excellent cellular integrity.


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